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2026, Vol: 10, Issue: 1
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Original Article
Online Published: 14 Nov 2025
 


Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department

Feras Ahmed Aljarri, Waleed Khalid Moosa, Hadi Abdulkarim Al Nas, Mahdi Abdulwahed Alsaeed, Sarah Albasha, Dunya Alfaraj, Abdullah Maher Felemban, Fatimah Alawami, Abdullah Alali, Abdulmonem Alsaleh.


Abstract
Objective:
This study aimed to assess the genome-wide DNA methylation profile associated with atherosclerotic disease and to identify differentially methylated sites associated with clinical features of plaque instability.
Methods:
Using silico analysis, four publicly available 850K and 450K methylation array datasets were analyzed to identify consistently differentially methylated positions in atherosclerosis. Based on the highest methylation difference (top 20%) and significance (p-value ≤0.001), differentially methylated positions were specified.
Results:
By analyzing four publicly available methylation datasets, 506 differentially methylated genes linked to atherosclerosis were detected. Moreover, a comparative analysis between all the datasets determined four shared differentially methylated genes in only two datasets: ODF2, MTRF1L, HMCN1, and GMDS. However, further analysis revealed that only one gene, which is ODF2, exhibited a significant increase in methylation levels within the promoter area, while the others were insignificant.
Conclusion:
While further validation is required, this study provided a comprehensive methylation profile in atherosclerotic patients and highlighted the novel identification of ODF2 hypomethylation as a potential biomarker for plaque instability.

Key words: Atherosclerosis, genes, DNA methylation, promoter area, stroke


 
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How to Cite this Article
Pubmed Style

Aljarri FA, Moosa WK, Nas HAA, Alsaeed MA, Albasha S, Alfaraj D, Felemban AM, Alawami F, Alali A, Alsaleh A. Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department. IJMDC. 2025; 9(10): 2430-2442. doi:10.24911/IJMDC.51-1759607891

Web Style

Aljarri FA, Moosa WK, Nas HAA, Alsaeed MA, Albasha S, Alfaraj D, Felemban AM, Alawami F, Alali A, Alsaleh A. Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department. https://www.ijmdc.com/?mno=288257 [Access: December 09, 2025]. doi:10.24911/IJMDC.51-1759607891

AMA (American Medical Association) Style

Aljarri FA, Moosa WK, Nas HAA, Alsaeed MA, Albasha S, Alfaraj D, Felemban AM, Alawami F, Alali A, Alsaleh A. Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department. IJMDC. 2025; 9(10): 2430-2442. doi:10.24911/IJMDC.51-1759607891


Vancouver/ICMJE Style

Aljarri FA, Moosa WK, Nas HAA, Alsaeed MA, Albasha S, Alfaraj D, Felemban AM, Alawami F, Alali A, Alsaleh A. Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department. IJMDC. (2025), [cited December 09, 2025]; 9(10): 2430-2442. doi:10.24911/IJMDC.51-1759607891


Harvard Style

Aljarri, F. A., Moosa, . W. K., Nas, . H. A. A., Alsaeed, . M. A., Albasha, . S., Alfaraj, . D., Felemban, . A. M., Alawami, . F., Alali, . A. & Alsaleh, . A. (2025) Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department. IJMDC, 9 (10), 2430-2442. doi:10.24911/IJMDC.51-1759607891


Turabian Style

Aljarri, Feras Ahmed, Waleed Khalid Moosa, Hadi Abdulkarim Al Nas, Mahdi Abdulwahed Alsaeed, Sarah Albasha, Dunya Alfaraj, Abdullah Maher Felemban, Fatimah Alawami, Abdullah Alali, and Abdulmonem Alsaleh. 2025. Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department. International Journal of Medicine in Developing Countries, 9 (10), 2430-2442. doi:10.24911/IJMDC.51-1759607891


Chicago Style

Aljarri, Feras Ahmed, Waleed Khalid Moosa, Hadi Abdulkarim Al Nas, Mahdi Abdulwahed Alsaeed, Sarah Albasha, Dunya Alfaraj, Abdullah Maher Felemban, Fatimah Alawami, Abdullah Alali, and Abdulmonem Alsaleh. "Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department." International Journal of Medicine in Developing Countries 9 (2025), 2430-2442. doi:10.24911/IJMDC.51-1759607891


MLA (The Modern Language Association) Style

Aljarri, Feras Ahmed, Waleed Khalid Moosa, Hadi Abdulkarim Al Nas, Mahdi Abdulwahed Alsaeed, Sarah Albasha, Dunya Alfaraj, Abdullah Maher Felemban, Fatimah Alawami, Abdullah Alali, and Abdulmonem Alsaleh. "Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department." International Journal of Medicine in Developing Countries 9.10 (2025), 2430-2442. Print. doi:10.24911/IJMDC.51-1759607891


APA (American Psychological Association) Style

Aljarri, F. A., Moosa, . W. K., Nas, . H. A. A., Alsaeed, . M. A., Albasha, . S., Alfaraj, . D., Felemban, . A. M., Alawami, . F., Alali, . A. & Alsaleh, . A. (2025) Epigenetic signature in atherosclerosis: a novel discovery of ODF2 methylation linked to unstable plaque and stroke risk stratification in the Emergency Department. International Journal of Medicine in Developing Countries, 9 (10), 2430-2442. doi:10.24911/IJMDC.51-1759607891




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