Original Research
IJMDC. 2018; 2(January 2018): 38-46
Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.
Authors: Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady.
ABSTRACT
IL-17 is a proinflammatory cytokine produced by T helper cells and is involved in the recruitment of white blood cells during the formation of granuloma induced by Schistosoma eggs. The aim of the present study was to examine the formation of in vitro granuloma induced by Schistosoma mansoni soluble egg antigen (SEA) after neutralization of IL-17 using Anti-IL-17 antibody. Peripheral blood mononuclear cells (PBMCs) were isolated from 20 S. mansoni monoinfected, 50 S. mansoni/Hepatitis C virus (HCV) coinfected patients and 25 healthy individuals. The isolated PBMCs were used for in vitro granuloma induction using S. mansoni SEA conjugated polyacrylamide beads. Different concentrations of Anti-IL-17 (0.5, 1, 2 or 4 ng/ml) were added to the cultured granuloma at the first day then the sizes of granuloma were assessed by granuloma index (GI) after 3, 7 or 14 days. Results revealed that, Anti-IL-17 at a concentration of 2 ng/ml optimally reduced the GI of the granuloma formed using PBMCs isolated from S. mansoni monoinfected, S. mansoni/HCV coinfected patients or healthy individuals after all the experimental time intervals. In conclusion, IL-17 blocking with the neutralizing Anti-IL-17 antibody promotes a marked reduction in the sizes of granuloma induced by S. mansoni SEA.
Keywords:
Peripheral blood mononuclear cells, Th-17 cells, IL-17 cytokine, Schistosoma mansoni, granuloma, Anti-IL-17 antibody.
Authors
Asmaa Ezz
Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles
Irene S Gmail
Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles
Fatma Elzahraa H Salem
Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles
Rabab Salama
Department of Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt
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Soad Nady
Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt
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Correspondence to:
Soad Nady. Soad Nady, Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt; soadnady@science.helwan.edu.eg
Publication history
Received 14 Feb 2018
Accepted 14 Mar 2018
Published in print 05 May 2018
Published online 05 May 2018
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Export to EndNote Export to refManPubmed Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. 2018; 2(January 2018): 38-46. doi:10.24911/IJMDC.2.1.7
Web Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. https://www.ijmdc.com/?mno=291092 [Access: May 28, 2023]. doi:10.24911/IJMDC.2.1.7
AMA (American Medical Association) Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. 2018; 2(January 2018): 38-46. doi:10.24911/IJMDC.2.1.7
Vancouver/ICMJE Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. (2018), [cited May 28, 2023]; 2(January 2018): 38-46. doi:10.24911/IJMDC.2.1.7
Harvard Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady (2018) Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC, 2 (January 2018), 38-46. doi:10.24911/IJMDC.2.1.7
Turabian Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. 2018. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. International Journal of Medicine in Developing Countries, 2 (January 2018), 38-46. doi:10.24911/IJMDC.2.1.7
Chicago Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. "Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.." International Journal of Medicine in Developing Countries 2 (2018), 38-46. doi:10.24911/IJMDC.2.1.7
MLA (The Modern Language Association) Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. "Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.." International Journal of Medicine in Developing Countries 2.January 2018 (2018), 38-46. Print. doi:10.24911/IJMDC.2.1.7
APA (American Psychological Association) Style
Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady (2018) Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. International Journal of Medicine in Developing Countries, 2 (January 2018), 38-46. doi:10.24911/IJMDC.2.1.7
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