E-ISSN 1658-7820
 

Original Research 


Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady.

Abstract
IL-17 is a proinflammatory cytokine produced by T helper cells and is involved in the recruitment of white blood cells during the formation of granuloma induced by Schistosoma eggs. The aim of the present study was to examine the formation of in vitro granuloma induced by Schistosoma mansoni soluble egg antigen (SEA) after neutralization of IL-17 using Anti-IL-17 antibody. Peripheral blood mononuclear cells (PBMCs) were isolated from 20 S. mansoni monoinfected, 50 S. mansoni/Hepatitis C virus (HCV) coinfected patients and 25 healthy individuals. The isolated PBMCs were used for in vitro granuloma induction using S. mansoni SEA conjugated polyacrylamide beads. Different concentrations of Anti-IL-17 (0.5, 1, 2 or 4 ng/ml) were added to the cultured granuloma at the first day then the sizes of granuloma were assessed by granuloma index (GI) after 3, 7 or 14 days. Results revealed that, Anti-IL-17 at a concentration of 2 ng/ml optimally reduced the GI of the granuloma formed using PBMCs isolated from S. mansoni monoinfected, S. mansoni/HCV coinfected patients or healthy individuals after all the experimental time intervals. In conclusion, IL-17 blocking with the neutralizing Anti-IL-17 antibody promotes a marked reduction in the sizes of granuloma induced by S. mansoni SEA.

Key words: Peripheral blood mononuclear cells, Th-17 cells, IL-17 cytokine, Schistosoma mansoni, granuloma, Anti-IL-17 antibody.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Asmaa Ezz
Articles by Irene S. Gmail
Articles by Fatma Elzahraa H. Salem
Articles by Rabab Salama
Articles by Soad Nady
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. 2018; 2(January 2018): 38-46. doi:10.24911/IJMDC.2.1.7


Web Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. https://www.ijmdc.com/?mno=291092 [Access: October 02, 2023]. doi:10.24911/IJMDC.2.1.7


AMA (American Medical Association) Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. 2018; 2(January 2018): 38-46. doi:10.24911/IJMDC.2.1.7



Vancouver/ICMJE Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. (2018), [cited October 02, 2023]; 2(January 2018): 38-46. doi:10.24911/IJMDC.2.1.7



Harvard Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady (2018) Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC, 2 (January 2018), 38-46. doi:10.24911/IJMDC.2.1.7



Turabian Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. 2018. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. International Journal of Medicine in Developing Countries, 2 (January 2018), 38-46. doi:10.24911/IJMDC.2.1.7



Chicago Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. "Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.." International Journal of Medicine in Developing Countries 2 (2018), 38-46. doi:10.24911/IJMDC.2.1.7



MLA (The Modern Language Association) Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. "Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.." International Journal of Medicine in Developing Countries 2.January 2018 (2018), 38-46. Print. doi:10.24911/IJMDC.2.1.7



APA (American Psychological Association) Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady (2018) Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. International Journal of Medicine in Developing Countries, 2 (January 2018), 38-46. doi:10.24911/IJMDC.2.1.7





Most Viewed Articles
  • Marginal adaptation of fixed prosthodontics
    Shahad Mohammed Halawani, Sahar Amer Al-Harbi
    IJMDC. 2017; 1(2): 78-84
    » Abstract » doi: 10.24911/IJMDC.1.2.7

  • Parasites in central nervous system
    Amal Ahmed Mohamed, Asmaa Mohamed Mansour
    IJMDC. 2017; 1(2): 72-77
    » Abstract » doi: 10.24911/IJMDC.1.2.6

  • Non-diabetic retinopathy: a case report
    Hassan F. Alkwikbi, Nabil Mamon Abdelfattah, Haithem Mamon Abdelfattah
    IJMDC. 2017; 1(1): 33-37
    » Abstract » doi: 10.24911/IJMDC.1.1.6

  • Vitamin D deficiency among high school female students in Riyadh
    Sahar Mohsen Aldhafeeri, Fatemah Ibraheem Alrawaji, Abrar Mubarak Algharbi, Alanoud Abdullah Alhessan, Ayat Sami Qabaja, Ghaleah Abdulaziz Alnassar, Ghaliah Ofayn Alsulami, Mashael Belal Alrubaian, Norah Abdulaziz Alhatim, Salehah Salih Alotaibi, Zahra Ali Alkhamees
    IJMDC. 2017; 1(1): 18-23
    » Abstract » doi: 10.24911/IJMDC.1.1.4

  • Prognosis of chronic complications of diabetes mellitus (DM) after multiple events of diabetic ketoacidosis (DKA)
    Baraa Faiez Rajab, Anwar Essa Alamrim, Ali Essa Alamri
    IJMDC. 2019; 3(5): 474-479
    » Abstract » doi: 10.24911/IJMDC.51-1546551993

  • Most Downloaded
  • Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.
    Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady
    IJMDC. 2018; 2(January 2018): 38-46
    » Abstract » doi: 10.24911/IJMDC.2.1.7

  • Vitamin D deficiency among high school female students in Riyadh
    Sahar Mohsen Aldhafeeri, Fatemah Ibraheem Alrawaji, Abrar Mubarak Algharbi, Alanoud Abdullah Alhessan, Ayat Sami Qabaja, Ghaleah Abdulaziz Alnassar, Ghaliah Ofayn Alsulami, Mashael Belal Alrubaian, Norah Abdulaziz Alhatim, Salehah Salih Alotaibi, Zahra Ali Alkhamees
    IJMDC. 2017; 1(1): 18-23
    » Abstract » doi: 10.24911/IJMDC.1.1.4

  • Antibiotic Resistance Crisis
    Nuha Saad Mobarki, Buthaina Abdullah Almerabi, Ahmad Hattan Hattan
    IJMDC. 2019; 3(6): 561-564
    » Abstract » doi: 10.24911/IJMDC.51-1549060699

  • The knowledge of mothers about children malnutrition and associated factors
    Anas Abdulrahman Aljohani, Mujahid Abdulrahman Aljohani
    IJMDC. 2020; 4(1): 7-11
    » Abstract » doi: 10.24911/IJMDC.51-1541620358

  • Knowledge and attitude of caregivers of cerebral palsy children in Riyadh city
    Abdulaziz Alruwaished, Baleegh Ali, Lubna Abdullah Alhowaimil , Abaraa adullah Alhowaimil, Najd Abdullah Alhowaimil, Aminah Alessa
    IJMDC. 2020; 4(1): 12-17
    » Abstract » doi: 10.24911/IJMDC.51-1547833051

  • Most Cited Articles