Original Research

IJMDC. 2018; 2(1): 38-46

Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.

Authors: Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady.

ABSTRACT

IL-17 is a proinflammatory cytokine produced by T helper cells and is involved in the recruitment of white blood cells during the formation of granuloma induced by Schistosoma eggs. The aim of the present study was to examine the formation of in vitro granuloma induced by Schistosoma mansoni soluble egg antigen (SEA) after neutralization of IL-17 using Anti-IL-17 antibody. Peripheral blood mononuclear cells (PBMCs) were isolated from 20 S. mansoni monoinfected, 50 S. mansoni/Hepatitis C virus (HCV) coinfected patients and 25 healthy individuals. The isolated PBMCs were used for in vitro granuloma induction using S. mansoni SEA conjugated polyacrylamide beads. Different concentrations of Anti-IL-17 (0.5, 1, 2 or 4 ng/ml) were added to the cultured granuloma at the first day then the sizes of granuloma were assessed by granuloma index (GI) after 3, 7 or 14 days. Results revealed that, Anti-IL-17 at a concentration of 2 ng/ml optimally reduced the GI of the granuloma formed using PBMCs isolated from S. mansoni monoinfected, S. mansoni/HCV coinfected patients or healthy individuals after all the experimental time intervals. In conclusion, IL-17 blocking with the neutralizing Anti-IL-17 antibody promotes a marked reduction in the sizes of granuloma induced by S. mansoni SEA.

Keywords:
Peripheral blood mononuclear cells, Th-17 cells, IL-17 cytokine, Schistosoma mansoni, granuloma, Anti-IL-17 antibody.


View PDF
Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.


Authors
Asmaa Ezz
Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles

Irene S Gmail
Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles

Fatma Elzahraa H Salem
Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles

Rabab Salama
Department of Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt
PubMed articlesGoogle scholar articles

Soad Nady
Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles


Correspondence to:
Soad Nady. Soad Nady, Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt; soadnady@science.helwan.edu.eg

Publication history
Published online 01 Jan 1970
Received 14 Feb 2018
Accepted 14 Mar 2018
Published in print 05 May 2018

How to cite this article?

Export to EndNote Export to refMan

Pubmed Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. 2018; 2(1): 38-46. doi:10.24911/IJMDC.2.1.7


Web Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. http://www.ijmdc.com/?mno=291092 [Access: November 21, 2018]. doi:10.24911/IJMDC.2.1.7


AMA (American Medical Association) Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. 2018; 2(1): 38-46. doi:10.24911/IJMDC.2.1.7


Vancouver/ICMJE Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC. (2018), [cited November 21, 2018]; 2(1): 38-46. doi:10.24911/IJMDC.2.1.7


Harvard Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady (2018) Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. IJMDC, 2 (1), 38-46. doi:10.24911/IJMDC.2.1.7


Turabian Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. 2018. Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. International Journal of Medicine in Developing Countries, 2 (1), 38-46. doi:10.24911/IJMDC.2.1.7


Chicago Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. "Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.." International Journal of Medicine in Developing Countries 2 (2018), 38-46. doi:10.24911/IJMDC.2.1.7


MLA (The Modern Language Association) Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady. "Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.." International Journal of Medicine in Developing Countries 2.1 (2018), 38-46. Print. doi:10.24911/IJMDC.2.1.7


APA (American Psychological Association) Style

Asmaa Ezz, Irene S. Gmail, Fatma Elzahraa H. Salem, Rabab Salama, Soad Nady (2018) Anti-IL-17 markedly inhibited the in vitro granuloma induced by Schistosoma mansoni soluble egg antigen.. International Journal of Medicine in Developing Countries, 2 (1), 38-46. doi:10.24911/IJMDC.2.1.7


Share this article

Click the icon of the social media platform on which you would like to share this article.

Article Statistics

Viewed: 1066
Downloaded: 125
Cited: 0

Request permissions

If you wish to reuse any part or all of this article please contact the copyright holder, contact@ejmcr.com.

Copyright © 2018 Discover Publishing Group. All Rights Reserved.