Original Research

IJMDC. 2017; 1(2): 52-60

The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro

Authors: Soad Nady, Irene S Gmail, Fatma Elzahraa H Salem, Rabab Salama, Asmaa Ezz.

ABSTRACT

Background: Pollen and pollen products have a long history of use in traditional herbal medicine and have been reported to display a wide range of antimicrobial, antioxidative, anti-inflammatory, antioxidant, and hepato-protective activities. This study aimed to investigate the role of date palm pollen (DPP) ethanolic extract on the formation of granuloma induced by Schistosoma mansoni soluble egg antigen (SEA) in vitro. Methodology: Peripheral blood mononuclear cells (PBMCs) were isolated from 20 S. mansoni monoinfected, 50 Hepatitis C virus/S. mansoni coinfected patients and 25 healthy individuals. The isolated PBMCs were used for in vitro granuloma induction using SEA conjugated polyacrylamide beads. Different concentrations of DPP ethanolic extract (10 mg/mL, 20 mg/mL, 30 mg/mL, and 40 mg/mL) were added to the granuloma at the first day, then the sizes of granulomas were measured by granuloma index (GI) that was assessed by quantitation of cellular reactivity around the beads after 3, 7, and 14 days. Results: Treatment of granuloma induced by SEA conjugated polyacrylamide beads with the optimum concen-tration of DPP ethanolic extract (30 mg/mL) resulted in significant (P < 0.05) reduction of GI using PBMCs iso¨lated from Schistosoma monoinfected, hepatitis C virus/Schistosoma coinfected patients or healthy individuals at all the experimental time intervals. Conclusion: DPP suppressed the effect of SEA and prevented granuloma formation. The results of the present study suggest that DPP could be considered a promising source of new natural anti-inflammatory herbal med¨icine against the S. mansoni associated granulomatous inflammation.

Keywords:
Date palm pollen, Schistosoma mansoni, granuloma, soluble egg antigen.


View PDF
The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro


Authors
Soad Nady
Department of Zoology and Entomology, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles

Irene S Gmail
Department of Zoology and Entomology, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles

Fatma Elzahraa H Salem
Department of Zoology and Entomology, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles

Rabab Salama
Department of Zoology and Entomology, Faculty of Science, Helwan University, Egypt
PubMed articlesGoogle scholar articles

Asmaa Ezz
Department of Endemic Medicine and Hepatology, Faculty of Medicine, Cairo University, Egypt
PubMed articlesGoogle scholar articles


Correspondence to:
. Zoology and Entomology Department, Faculty of Science, Helwan University, Egypt.;

Publication history

How to cite this article?

Export to EndNote Export to refMan

Pubmed Style

Nady S, Gmail IS, Salem FEH, Salama R, Ezz A. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. IJMDC. 2017; 1(2): 52-60. doi:10.24911/IJMDC.1.2.3


Web Style

Nady S, Gmail IS, Salem FEH, Salama R, Ezz A. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. http://www.ijmdc.com/?mno=287573 [Access: September 21, 2018]. doi:10.24911/IJMDC.1.2.3


AMA (American Medical Association) Style

Nady S, Gmail IS, Salem FEH, Salama R, Ezz A. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. IJMDC. 2017; 1(2): 52-60. doi:10.24911/IJMDC.1.2.3


Vancouver/ICMJE Style

Nady S, Gmail IS, Salem FEH, Salama R, Ezz A. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. IJMDC. (2017), [cited September 21, 2018]; 1(2): 52-60. doi:10.24911/IJMDC.1.2.3


Harvard Style

Nady, S., Gmail, I. S., Salem, F. E. H., Salama, R. & Ezz, A. (2017) The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. IJMDC, 1 (2), 52-60. doi:10.24911/IJMDC.1.2.3


Turabian Style

Nady, Soad, Irene S Gmail, Fatma Elzahraa H Salem, Rabab Salama, and Asmaa Ezz. 2017. The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. International Journal of Medicine in Developing Countries, 1 (2), 52-60. doi:10.24911/IJMDC.1.2.3


Chicago Style

Nady, Soad, Irene S Gmail, Fatma Elzahraa H Salem, Rabab Salama, and Asmaa Ezz. "The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro." International Journal of Medicine in Developing Countries 1 (2017), 52-60. doi:10.24911/IJMDC.1.2.3


MLA (The Modern Language Association) Style

Nady, Soad, Irene S Gmail, Fatma Elzahraa H Salem, Rabab Salama, and Asmaa Ezz. "The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro." International Journal of Medicine in Developing Countries 1.2 (2017), 52-60. Print. doi:10.24911/IJMDC.1.2.3


APA (American Psychological Association) Style

Nady, S., Gmail, I. S., Salem, F. E. H., Salama, R. & Ezz, A. (2017) The ethanolic extract of date palm pollen significantly inhibited granuloma induced by Schistosoma mansoni soluble egg antigen in vitro. International Journal of Medicine in Developing Countries, 1 (2), 52-60. doi:10.24911/IJMDC.1.2.3


Share this article

Click the icon of the social media platform on which you would like to share this article.

Article Statistics

Viewed: 1911
Downloaded: 289
Cited: 0

Request permissions

If you wish to reuse any part or all of this article please contact the copyright holder, contact@ejmcr.com.

Copyright © 2018 Discover Publishing Group. All Rights Reserved.